1. Update the map of data set with Build37 Cox genetic map (B37) and save the new file as "Ishimori2004_B6x129_B37_Data.csv". This file is ready to be read into Rqtl; 2. Generate a description file of the project, it includes the general information, definitions of the phenotypes, description of the genotypes and information of the missing markers; 3. For the genotypes of this cross: (cM=centimorgan, bp=basepair) (1) Change the symbol (original data) of 129 allele from "0" to "A", B6 allele from "2" to "B", heterozygous allele from "1" to "H", missing genotypes from "=" to "-" (standard format in data sets of QTL archive) in B37 data file; (2) Marker "D1Mit406" misses bp position in both Cox map and MGI database. Primer sequences found in MGI, and their locations identified using primer-BLAST in NCBI followed by UCSC In-Silico PCR (it is highlighted with lime color in description file); (3) Marker "D14Mit75" and "D18Mit42" miss bp positions in both Cox map and MGI database. No primer sequences are available from MGI. We could not identify the B37 bp position for these 2 markers currently (this type of markers is highlighted with pale blue color in description file); (4) Five markers miss their bp positions in Cox genetic map, assign the position from current MGI database. This type of markers are highlighted with gold color and please see description file for details; (5) Some markers are at the same position (cM) on chromosome 10 in B37 Cox map; (6) This project only has female mice. Change the symbol of female mouse from "F" to "0" and save the changes in B37 data file; 4. For the phenotypes of the cross: (1) Change the letters from "F" to "0" (female mice) of trait "sex" in B37 data file; (2) The Rqtl program detects the errors of trait "pgm" (paternal grandma) in original data -- the 0/1 values for "pgm" appear to be switched. I switch the values from "0" to "1" and save the changes in B37 data file; (3) Remove two unnecessary traits "BMD_areal" and "BMD_spinal" from cross file; 5. Except the "csv" file mentioned above, I also saved the description file, original data, data with B37 map, data process and list of missing markers together as a big excel file ("Data_Description_B6x129_Ishimori2004.xlsx") (1st curating work is finished in 2009, files are updated on 12/1/2010, 1/13, 8/25/2011). 6. Information updated on 9/1/2011: Added a new related publication in description file; updated map from Female cM to build 37 sex-averaged cM; 7. Information updated on 10/26/2011: Generate genetic map plot, missing genotypes plot, map comparison plot, whole genome RF plot and problematic chromosome RF plots for B37 data. The last 2 markers on chr 7 (observed from RF plot) and the last marker on 17 might have problems (observed from map comparison plot). All quality control plots were saved in ÒQCReport_Ishimori2004.pdfÓ.